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Abstract

Effect of MST1 on Invasion and Migration of Colon Cancer through Mitogen-Activated Protein Kinase/Extracellular Signal-Regulated Kinase Signal Pathway

Author(s): Xu Wenmin, Tan Jingyu and Luo Hanyi*
Health Examination and Tumor Screening Center, Chongqing University Cancer Hospital, Chongqing 400030, China

Correspondence Address:
Luo Hanyi, Health Examination and Tumor Screening Center, Chongqing University Cancer Hospital, Chongqing 400030, China, E-mail: 243482159@qq.com


To explore the effect of mammalian STE20 like protein kinase 1 on colon cancer invasion and metastasis through mitogen-activated protein kinase/extracellular signal-regulated kinase signaling pathway. Three groups of cells were set up; blank control group, colon cancer group and mammalian STE20 like protein kinase 1 overexpression group. The proliferation ability of the three groups of cells was assessed using cell counting kit 8, protein expression was detected using Western blot, the expression level of relevant messenger ribonucleic acid was determined using quantitative polymerase chain reaction, and the migration and invasion ability of the cells was evaluated using Transwell. The expression level of B-cell lymphoma 2 was markedly significantly lower reduced than that of colon cancer group. The relative expression of mitogen-activated protein kinase, extracellular regulated kinase messenger ribonucleic acid and protein in the colon cancer group was markedly significantly higher than that in the blank control group; the relative expression of mitogen-activated protein kinase, extracellular regulated kinase messenger ribonucleic acid and protein in the mammalian STE20 like protein kinase 1 overexpression group was markedly significantly lower reduced than that in the colon cancer group. Overexpression of mammalian STE20 like protein kinase 1 can block the mitogen-activated protein kinase-extracellular regulated kinase signal transduction pathway, reduce the viability of colon cancer cells, restrain the proliferation growth, migration and invasion of colon cancer cells, and induce apoptosis of colon cancer cells, thus ultimately contributing to the establishment of a theoretical foundation for the development of targeted therapies for colon cancer.

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