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Abstract

Effect of Sex Determining Region Y-Box 2 on the Biological Characteristics of Breast Cancer Cells and its Clinical Significance

Author(s): Lianji Feng, Jingmin Wang, Zhenling Ji, Xiaoli Le and Heng Zhang*
Department of General Surgery, Nanjing Lishui People’s Hospital, Zhongda Hospital Lishui Branch, Southeast University, 1Department of General Surgery, Zhongda Hospital, Southeast University, 2Department of Pathology, Nanjing Lishui People’s Hospital, Zhongda Hospital Lishui Branch, Southeast University, Nanjing, Jiangsu 211200, China

Correspondence Address:
Heng Zhang, Department of General Surgery, Nanjing Lishui People’s Hospital, Zhongda Hospital Lishui Branch, Southeast University, Nanjing, Jiangsu 211200, China


To investigate the effects of the sex determining region Y-box 2 gene silencing on biological characteristics and the mitogen-activated protein kinase kinase kinase kinase 4 signal pathway of breast cancer cells. Firstly, the expression of sex determining region Y-box 2 in breast cancer was detected by the immunohistochemical method and the relationship with clinicopathological features was analyzed. Then the sex determining region Y-box 2 silencing breast cancer cell line Michigan Cancer Foundation-7 was prepared, and the messenger ribonucleic acid and protein expression levels of sex determining region Y-box 2, c-Jun N-terminal kinase, p53 and tumor necrosis factor-alpha in the mitogen-activated protein kinase kinase kinase kinase 4 pathway were detected by quantitative polymerase chain reaction and western blotting. Cell proliferation, migration, invasion and apoptosis were detected by the Cell Counting Kit-8, scratch test, transwell test, and flow cytometry. After si-sex determining region Y-box 2 transfection, the expression of sex determining region Y-box 2, c-Jun N-terminal kinase, p53 and tumor necrosis factor-alpha decreased, cell growth was inhibited, cell proliferation, migration and invasion decreased significantly, and apoptosis increased, while si-sex determining region Y-box 2+ mitogen-activated protein kinase kinase kinase kinase 4 transfection could reverse the above trend. Sex determining region Y-box 2 silencing can activate the mitogen-activated protein kinase kinase kinase kinase 4 signal pathway, promote the proliferation, migration, and invasion of breast cancer cells and inhibit apoptosis.

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