Abstract

In this study, the effect of sheep nano antibody on the movement of human tissue flap muscle is studied. Firstly, the non-toxic effect of bivalent sheep nano antibody on mouse muscle cells is investigated and then the effect of the antibody on the movement of mouse tissue flap muscle is analyzed. In the repeated experiment of injecting bivalent sheep myostatin nano antibody into mice, 30 healthy Kunming mice of 1 mo old and weighing 15 g without specific pathogen are selected. When using 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyl tetrazolium bromide method to detect the myoblast toxicity of mouse immortalized mouse myoblast cell line C2C12, phosphate buffered saline control group, blank control group and the observation group with concentration of 10, 20, 30 and 60 μg/ml of bivalent nano antibody membrane proteome array are set respectively. In the repeated experiment of bivalent sheep myostatin nano antibody on mice, it is divided into phosphate buffered saline control group, monovalent group and bivalent group. Each animal in phosphate buffered saline group is injected with 30 μl phosphate buffered saline, each animal in monovalent group is injected with 30 μl (60 μg) monovalent sheep myostatin nano antibody and each animal in bivalent group is injected with 30 μl (60 μg) bivalent sheep myostatin nano antibody. The weight of the single hind limb of the bivalent sheep myostatin nano antibody group is higher than that of the monovalent sheep myostatin nano antibody group and the control group, and the muscle morphology of the hind limb of the bivalent sheep myostatin nano antibody group is significantly higher than that of the monovalent sheep myostatin nano antibody group and the control group, which is more plump. The results show that sheep nano antibody is non-toxic to human tissue flap muscle, which can promote the growth of human tissue flap muscle and enhance muscle movement ability.