Abstract
Effects of GLP-1 Analogue, Liraglutide on the Expression of Mouse Pre-osteoblast MC3T3-E1
Third Department of Orthopaedics, General Hospital of Ningxia Medical University, No. 804 South Shengli Street, Xingqing District, Yinchuan City, Ningxia Hui Autonomous Region, 750004, China
Correspondence Address:
Third Department of Orthopaedics, General Hospital of Ningxia Medical University, No. 804 South Shengli Street, Xingqing District, Yinchuan City, Ningxia Hui Autonomous Region, 750004, China, E-mail: jinqunhuagzh018@126.com
This investigation dealt with the investigation of the effects of GLP-1 analogue, liraglutide on the expression of mouse pre-osteoblast MC3T3-E1, MC3T3-E1 cells were cultured, passaged and frozen. Cell lines were cultured under different conditions and were separated into control group exposed to saline and groups 2, 3 and 4, which were exposed to 10-9 M, 10-8 M and 10-7 M liraglutide, respectively. Group 2 cells after 2 d of intervention were subjected to CCK-8 assay for cell proliferation, flow cytometry for cell apoptosis and Western blot for protein expression. The expression of osteoblast differentiation marker genes, GLP-1R, Runx2, OCN and ALP were detected using real-time fluorescence quantitative PCR 3, 6, 12 and 20 days after intervention. The results showed that liraglutide promoted proliferation of osteoblasts and the reduced early apoptotic rate of osteoblasts, which indicated that liraglutide could promote the proliferation and differentiation of mouse osteoblasts MC3T3-E1 and inhibited the apoptosis of cells.
