Abstract
Effects of Long Non-Coding RNA MALAT1 Induced by High Glucose on the Biological Characteristics in Stroke
Department of Rehabilitation, Liyuan Hospital, Tongji Medical College, Huazhong University of Science and Technology, Hubei 430077, China
Correspondence Address:
G. Xie, Department of Rehabilitation, Liyuan Hospital, Tongji Medical College, Huazhong University of Science and Technology, Hubei 430077, China, E-mail: xg6621g@163.com
To investigate the effect of high glucose-induced long non-coding ribonucleic acid metastasis associated lung adenocarcinoma transcript 1 on the biological properties of stroke cells. Rat brain micro vascular endothelial cells were cultured in vitroand divided into non-high glucose culture group and high glucose culture group. Long non-coding ribonucleic acid- metastasis associated lung adenocarcinoma transcript 1 was overexpressed and silenced by cell transfection, and the expression levels of long non-coding ribonucleic acid- metastasis associated lung adenocarcinoma transcript 1 and miR-181a-5p were detected by reverse transcription polymerase chain reaction under different culture methods. The Cell TiterBlue assay was also used to analyze the changes of cell viability after various transfection treatments; the double fluorophore reporter gene assay was used to verify the targeting effect of long non-coding ribonucleic acid- metastasis associated lung adenocarcinoma transcript 1 and miR-181a-5p. The results showed that non-high glucose cultured rat brain micro vascular endothelial cells significantly down-regulated the expression of Long non-coding ribonucleic acid- metastasis associated lung adenocarcinoma transcript 1 compared to high glucose cultured rat brain micro vascular endothelial cells, but the expression level of miR-181a-5p was significantly increased with statistically significant differences (p<0.05). Compared with the control group, the viability of rat brain micro vascular endothelial cells expression long non-coding ribonucleic acid- metastasis associated lung adenocarcinoma transcript 1 was significantly enhanced (p<0.05), while silencing long non-coding ribonucleic acid- metastasis associated lung adenocarcinoma transcript 1 significantly inhibit the viability of rat brain micro vascular endothelial cells (p<0.05). Reverse transcription polymerase chain reaction experiments showed that long non-coding ribonucleic acidmetastasis associated lung adenocarcinoma transcript 1 regulated the viability of rat brain micro vascular endothelial cells with miR-181a-5p expression levels at a positive correlation level, while dual fluorophore enzyme reporter gene assays suggested a region of complementary binding between long non-coding ribonucleic acid- metastasis associated lung adenocarcinoma transcript 1 and miR-181a-5p. Long noncoding ribonucleic acid- metastasis associated lung adenocarcinoma transcript 1 can achieve the effect on the activity of rat brain micro vascular endothelial cells under high glucose stimulation by regulating miR-181a-5p.
