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Abstract

Effects of Long Non-Coding RNA MALAT1 Induced by High Glucose on the Biological Characteristics in Stroke

Author(s): L. Wang, J. Q. Peng and G. Xie*
Department of Rehabilitation, Liyuan Hospital, Tongji Medical College, Huazhong University of Science and Technology, Hubei 430077, China

Correspondence Address:
G. Xie, Department of Rehabilitation, Liyuan Hospital, Tongji Medical College, Huazhong University of Science and Technology, Hubei 430077, China, E-mail: [email protected]


To investigate the effect of high glucose-induced long non-coding ribonucleic acid metastasis associated lung adenocarcinoma transcript 1 on the biological properties of stroke cells. Rat brain micro vascular endothelial cells were cultured in vitroand divided into non-high glucose culture group and high glucose culture group. Long non-coding ribonucleic acid- metastasis associated lung adenocarcinoma transcript 1 was overexpressed and silenced by cell transfection, and the expression levels of long non-coding ribonucleic acid- metastasis associated lung adenocarcinoma transcript 1 and miR-181a-5p were detected by reverse transcription polymerase chain reaction under different culture methods. The Cell TiterBlue assay was also used to analyze the changes of cell viability after various transfection treatments; the double fluorophore reporter gene assay was used to verify the targeting effect of long non-coding ribonucleic acid- metastasis associated lung adenocarcinoma transcript 1 and miR-181a-5p. The results showed that non-high glucose cultured rat brain micro vascular endothelial cells significantly down-regulated the expression of Long non-coding ribonucleic acid- metastasis associated lung adenocarcinoma transcript 1 compared to high glucose cultured rat brain micro vascular endothelial cells, but the expression level of miR-181a-5p was significantly increased with statistically significant differences (p<0.05). Compared with the control group, the viability of rat brain micro vascular endothelial cells expression long non-coding ribonucleic acid- metastasis associated lung adenocarcinoma transcript 1 was significantly enhanced (p<0.05), while silencing long non-coding ribonucleic acid- metastasis associated lung adenocarcinoma transcript 1 significantly inhibit the viability of rat brain micro vascular endothelial cells (p<0.05). Reverse transcription polymerase chain reaction experiments showed that long non-coding ribonucleic acidmetastasis associated lung adenocarcinoma transcript 1 regulated the viability of rat brain micro vascular endothelial cells with miR-181a-5p expression levels at a positive correlation level, while dual fluorophore enzyme reporter gene assays suggested a region of complementary binding between long non-coding ribonucleic acid- metastasis associated lung adenocarcinoma transcript 1 and miR-181a-5p. Long noncoding ribonucleic acid- metastasis associated lung adenocarcinoma transcript 1 can achieve the effect on the activity of rat brain micro vascular endothelial cells under high glucose stimulation by regulating miR-181a-5p.

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