Abstract

The aim of this study was to explore the effect of long non-coding RNA TRIM52 antisense RNA 1 on the proliferation, apoptosis and radiosensitivity of colorectal cancer cells and its possible mechanism. Real-time quantitative reverse transcription polymerase chain reaction method was used to detect the expression of long non-coding RNA TRIM52 antisense RNA 1 and microRNA-361-5p in adjacent tissues, colorectal cancer tissues and human normal colorectal mucosal cell lines, fetal human cells and human colorectal cancer cell lines. The transplanted tumor experiment in nude mice was used to detect the influence of the expression changes of long non-coding RNA TRIM52 antisense RNA 1 and microRNA-361-5p on the transplanted tumor growth. The expression of long non-coding RNA TRIM52 antisense RNA 1 in colorectal cancer tissues and cell lines was increased, while the expression of microRNA-361-5p was decreased (p<0.05). After transfection of small interfering long non-coding RNA TRIM52 antisense RNA 1 or microRNA-361-5p mimics, cell viability and cell survival score were decreased, the number of cell clone formation was decreased and cell apoptosis rate was increased (p<0.05). Transfection of small interfering long non-coding RNA TRIM52 antisense RNA 1 could inhibit the growth of transplanted tumors in nude mice. Long non-coding RNA TRIM52 antisense RNA 1 could target the microRNA-361-5p expression. The expression of long non-coding RNA TRIM52 antisense RNA 1 could inhibit colorectal cancer cell proliferation and clone formation and promote cell apoptosis by targeted regulation of microRNA-361-5p expression and could enhance the radiosensitivity of colorectal cancer cells.