Abstract
Effects of Transforming Growth Factor-Beta/SMAD Signal Mediated by Poly-L-Lactic Acid on Biological Behavior and Collagen Expression of Dermal Fibroblasts
Department of Plastic Surgery, Tongde Hospital of Zhejiang Province, Zhejiang Province 310012, 1The Second Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, Zhejiang Province 310053, China
Correspondence Address:
Lijuan Peng, Department of Plastic Surgery, Tongde Hospital of Zhejiang Province, Zhejiang Province 310012, China, E-mail: wuhgzg19432@163.com
To exam the effects of transforming growth factor-beta1/suppressor of mothers against decapentaplegic signal mediated by poly L-lactic acid on biological behavior and collagen expression of dermal fibroblasts. Dermal fibroblasts Hs60 were treated with different concentrations of poly L-lactic acid. Quantitative polymerase chain reaction was used to detect the collagen type I alpha 1, collagen type I alpha 2, elastin, matrix metalloproteinases-1, tissue inhibitors of metalloproteinases-1 and tissue inhibitors of metalloproteinases-2, messenger ribonucleic acid level; procollagen synthesis was analyzed using a type I prolactin inducible protein enzyme immunoassay kit, and the amount of proteins associated to the transforming growth factorbeta 1/suppressor of mothers against decapentaplegic signal pathway was measured using Western blot. The procollagen products in the high poly L-lactic acid intervention group (group C) was raised than that in the low- poly L-lactic acid intervention group (group B) and the control group (group A). The collagen type I alpha 1 and collagen type I alpha 2 protein in the group C were increased than those in the lowpoly L-lactic acid intervention group and the group A. The elastin, metalloproteinases-1, tissue inhibitors of metalloproteinases-1 and tissue inhibitors of metalloproteinases-2 messenger ribonucleic acid in group C was raised than that in low-poly L-lactic acid intervention group and group A, while the metalloproteinases-1, messenger ribonucleic acid in group B was reduced than that in group C and group A. The transforming growth factor-beta1 and suppressor of mothers against decapentaplegic messenger ribonucleic acid in group C was raised than that in low-poly L-lactic acid intervention group and group A. The transforming growth factor-beta1 and suppressor of mothers against decapentaplegic protein in the group C were increased than those in the low-poly L-lactic acid intervention group and the group A. Poly L-lactic acid stimulates collagen expression and synthesis in dermal fibroblasts by activating transforming growth factor-beta1/suppressor of mothers against decapentaplegic signal pathway.
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