Abstract
Efficacy of Tiam1 in Modulation of Lymphomas Tumor Invasiveness in Oral Squamous Cell Carcinoma
Department of Stomatology, School and Hospital of Stomatology, Tianjin Medical University, Qixiangtai, Heping, Tianjin 300070, 1Department of Immunology, Second Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Zhenli, Tianjin 300150, 2Department of Immunology, Key Laboratory of Immune Microenvironment and Disease of the Ministry of Education, Tianjin Medical University, Qixiangtai, Heping, Tianjin 300070, 3Department of Clinical Laboratory, Tianjin Dongli Hospital, Dongli, Tianjin 300399, China
Correspondence Address:
H. L. Guo, Department of Stomatology, School and Hospital of Stomatology, Tianjin Medical University, Qixiangtai, Heping, Tianjin 300070, China, E-mail: hguo@tmu.edu.cn
To better understand the functions of T-cell lymphoma invasion and metastasis-inducing factor 1 for oral squamous cell carcinoma patients with lymphoma metastasis, we performed this study. This study was a retrospective study using clinical recruited oral squamous cell carcinoma individuals. The differentially expressed messenger ribonucleic acid and micro ribonucleic acid for oral squamous cell carcinoma patients with lymphoma metastasis was generated compared with conventional oral squamous cell carcinoma. The primary differentially expressed messenger ribonucleic acids and differentially expressed micro ribonucleic acids were further investigated in human lymphomas cell line Rec-1. The oral squamous cell carcinoma patients with lymphoma metastasis demonstrated 268 differentially expressed messenger ribonucleic acids and 50 differentially expressed micro ribonucleic acids. The T-cell lymphoma invasion and metastasis-inducing factor 1 gene and micro ribonucleic acid-30a was the primary differentially expressed messenger ribonucleic acids and differentially expressed micro ribonucleic acids respectively. The concentration of T-cell lymphoma invasion and metastasis-inducing factor 1 in the peripheral blood of oral squamous cell carcinoma patients with lymphoma metastasis was dramatically increased to 83.2±19.8 ng/ml, compared with 34.8±11.6 ng/ml in conventional oral squamous cell carcinoma patients (p<0.05). The T-cell lymphoma invasion and metastasisinducing factor 1 was a direct target for micro ribonucleic acid-30a. T-cell lymphoma invasion and metastasisinducing factor 1 activated the Wingless-related integration site and c-Jun N-terminal kinase signaling pathways based on enrichment analysis and cellular functional experiments. Rec-1 cells transfected with T-cell lymphoma invasion and metastasis-inducing factor 1, small interfering ribonucleic acid significantly reduced the colony formation, cell proliferation and cell invasion while elevated cell apoptosis. This study hypothesized T-cell lymphoma invasion and metastasis-inducing factor 1 as a key regulator for oral squamous cell carcinoma with lymphoma metastasis, which was modulated by micro ribonucleic acid-30a.
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