Abstract
Astragalus Polysaccharide Regulates Proliferation, Migration and Invasion of Lung Cancer Cells via Janus Kinase/Signal Transducer and Activator of Transcription Signaling Pathway
Department of Respiratory Medicine, Jiangnan Hospital Affiliated to Zhejiang University of Traditional Chinese Medicine, Hangzhou, Zhejiang Province 311200, China
Correspondence Address:
J. Cao, Department of Respiratory Medicine, Jiangnan Hospital Affiliated to Zhejiang University of Traditional Chinese Medicine, Hangzhou, Zhejiang Province 311200, China, E-mail: cao.jianbiao@163.com
To evaluate the regulatory effects of Astragalus polysaccharide on the proliferation, migration and invasion of lung cancer cells via the Janus kinase/signal transducer and activator of transcription signaling pathway. The inhibitory effects of treatment with Astragalus polysaccharide at different concentrations (0, 0.5, 1.0, 2.0, 4.0, 8.0 and 16.0 mg/ml) for different time (24, 48 and 72 h) on the proliferation of adenocarcinomic human alveolar basal epithelial cells in the logarithmic growth phase were detected by methyl thiazolyl tetrazolium assay and its inhibitory effects on the migration and invasion of adenocarcinomic human alveolar basal epithelial cells were determined by wound healing assay and Transwell migration and invasion assays respectively. In contrast with control group, the proliferation of adenocarcinomic human alveolar basal epithelial cells treated with different concentrations of Astragalus polysaccharide for 24, 48 and 72 h was remarkably inhibited in a dose and time dependent manner under the same drug concentration, longer the action time, higher the proliferation inhibition rate of adenocarcinomic human alveolar basal epithelial cells; under the same action time, higher the drug concentration, higher the proliferation inhibition rate of adenocarcinomic human alveolar basal epithelial cells. The number of migrating cells dropped significantly in Astragalus polysaccharide groups (p<0.05), which was more obvious with the increase of Astragalus polysaccharide concentration (p<0.05). Astragalus polysaccharide can dramatically inhibit the proliferation of lung cancer adenocarcinomic human alveolar basal epithelial cells in a concentration and time-dependent manner and also effectively suppress their migration and invasion. The mechanism may be related to its ability to up-regulate the protein expressions of Src homology region 2 containing protein tyrosine phosphatase 2 and suppressor of cytokine signaling 3 and inhibit the over-activation of the Janus kinase/signal transducer and activator of transcription signaling pathway in lung cancer adenocarcinomic human alveolar basal epithelial cells.
