Abstract

The effective polysaccharide differentiation-inducing factor-1 was obtained by separating the traditional Chinese medicine Duchesnea indica. In vitro, differentiation-inducing factor-1 significantly inhibited the migration and invasion of HT-29 cells; it can significantly inhibit the expression of transforming growth factor-beta induced the decrease of E-cadherin and inhibited the increase of N-cadherin expression; at the same time, it can significantly inhibit the phosphorylation process of suppressor of mothers against decapentaplegic 2 and suppressor of mothers against decapentaplegic 3 and reduce the protein expression. In in vivo experiment, the number of liver metastatic nodules and the volume of tumor tissue in the administration group (differentiation-inducing factor-1-L and differentiation-inducing factor-1-H, positive) were lower than those in the model group (p<0.05). Differentiation-inducing factor-1 can improve the liver tissue of nude mice, maintain the complete structure and reduce the number of heterosexual nuclei. Western blot showed that the expression of E-cadherin increased significantly and the expression of N-cadherin decreased under the action of differentiation-inducing factor-1. Differentiation-inducing factor-1 can significantly inhibit the migration and invasion of HT-29 cells in vitro. In vivo experiments further prove that differentiation-inducing factor-1 can reduce the number and volume of tumors in vivo, and inhibit the metastasis of HT-29 cells in vivo. The mechanism may be through suppressor of mothers against decapentaplegic pathway.