Abstract
In Vitro and In Vivo Regulation of miR-423-5p Genes Associated with Retinoid-Interferon-Induced Mortality-19 by Inhibiting Hepatocellular Carcinoma via Competitive Endogenous Ribonucleic Acid Pathway
Department of Liver Transplantation, Tianjin First Center Hospital, Tianjin, Nankai 300384, 1Department of Medicine, Nankai University, Tianjin, Nankai 300071, China
Correspondence Address:
W. Jiang, Department of Liver Transplantation, Tianjin First Center Hospital, Tianjin, Nankai 300384, 1Department of Medicine, Nankai University, Tianjin, Nankai 300071, China, E-mail: jiangwentao_tjfch@126.com
The objective of this study was to explore the role of miR-423-5p in the pathogenesis of liver cancer. Hepatocellular carcinoma cells were transfected with miR-423-5p mimics or miR-423-5p inhibitors, respectively. In this paper, the effect of miR-423-5p on the proliferation and migration of hepatocellular carcinoma cells was identified. Detection of miR-423-5p and genes associated with retinoid-interferon-induced mortality-19 messenger ribonucleic acid by Western blot and quantitative reverse transcription polymerase chain reaction. We verified the relationship of miR-423-5p with genes associated with retinoid-interferon-induced mortality-19 using double luciferase assay. The outcomes indicated that the expression of miR-423- 5p was up-regulated in human hepatoma tissues and cells. Moreover, reducing miR-423-5p can inhibit the proliferation and migration ability of hepatoma cells. Mechanistically, the experimental data proves that genes associated with retinoid-interferon-induced mortality-19 are the direct target of miR-423-5p in hepatoma cells. At the same time, rescue experiments verified that over-expression of genes associated with retinoid-interferon-induced mortality-19 may counteract the ability of miR-423-5p to enhance the proliferation and metastasis of hepatoma. Further, the outcomes showed an abnormal down-regulation of the cell cycle inhibitor p21 in hepatocellular carcinoma cells, which was reversed by the reduction of miR-423-5p. It is suggested that miR-423-5p may enhance the proliferation and metastasis of hepatoma cells by targeting genes associated with retinoid-interferon-induced mortality-19.
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