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Abstract

Estimation of duloxetine hydrochloride in pharmaceutical formulations by RP-HPLC method

Author(s): Sejal K Patel, NJ Patel, KM Patel, PU Patel, BH Patel
S. K. Patel college of Pharmaceutical Education and Research, Ganpat University, Kherava-382 711, India

Correspondence Address:
Sejal K Patel S. K. Patel college of Pharmaceutical Education and Research, Ganpat University, Kherava-382 711 India E-mail: skpatel_2@rediffmail.com


Simple, specific, accurate and precise method, namely, reverse phase high performance liquid chromatography was developed for estimation of duloxetine HCl in pharmaceutical formulations. For the high performance liquid chromatography method, Phenomenox C-18, 5 µm column consisting of 250×4.6 mm i.d. in isocratic mode, with mobile phase containing 0.01M 5.5 pH phosphate buffer: acetonitrile (60:40 v/v) and final pH adjust to 5.5±0.02 with phosphoric acid was used. The flow rate was 1.2 ml/min and effluent was monitored at 231 nm. The retention time was 5.61 min. The method was validated in terms of linearity, accuracy and precision. The linearity curve was found to be linear over 0.25-4 µg/ml. The limit of detection and limit of quantification were found to be 0.10 and 0.25 µg/ml respectively. The proposed method was successfully used to determine the drug content of marketed formulations.

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Citations : 66710

Indian Journal of Pharmaceutical Sciences received 66710 citations as per google scholar report