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Estimation of Phytochemical Content and Antioxidant Activity of Some Selected Traditional Indian Medicinal Plants

Author(s): Nilima S Rajurkar, SM Hande

The powder samples and methanol extract of 11 medicinal plants were subjected to analysis of proximate composition and measurement of antioxidant activity. Different parameters studied include phenolic contents, moisture, ash, crude fiber, fats and waxes. The assays employed were ferric reducing antioxidant power, trolox equivalent antioxidant capacity and scavenging effect on the 1,1-diphenyl-2-picrylhydrazyl free radical. Results obtained indicate that the antioxidant potential varied significantly from plant to plant. The total phenolic contents were determined spectrophotometrically using Folin-Ciocalteu reagent. Significant correlation is observed between ferric reducing antioxidant power and phenolic contents (R2= 0.96). These findings show that the polyphenolic constituents in the extracts are responsible for free radical scavenging capacity.

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