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Abstract
Estimation of Pramipexole Dihydrochloride in Tablet Formulation by the Developed Reverse Phase High Performance Liquid Chromatography Method and its Validation
Author(s): P. Venkatesh and S. Gayatri*
Faculty of Pharmacy, Sri Ramachandra Institute of Higher Education and Research, Chennai, Tamil Nadu 600116, India
Correspondence Address:
S. Gayatri, Faculty of Pharmacy, Sri Ramachandra Institute of Higher Education and Research, Chennai, Tamil Nadu 600116, India
Faculty of Pharmacy, Sri Ramachandra Institute of Higher Education and Research, Chennai, Tamil Nadu 600116, India
Correspondence Address:
S. Gayatri, Faculty of Pharmacy, Sri Ramachandra Institute of Higher Education and Research, Chennai, Tamil Nadu 600116, India
A simple, rapid isocratic reverse phase high performance liquid chromatography method was developed and validated for the determination of pramipexole dihydrochloride in tablet dosage form as per International Council on Harmonisation guidelines. In this method the chromatographic separation was carried out using C18 column with ultraviolet detector set at 264 nm. The eluent consisted of acetonitrile:phosphate buffer (pH 4.0) (70:30 by volume) with a flow rate of 1 ml/min. The method showed linear response with the retention time of 2.28 min. The main advantage of the developed method is its cost effectiveness and shortened run time. Hence this method can be applied for routine analysis of pramipexole in pharmaceutical dosage form.
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