All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Abstract

Exploration of the Expression of Circulating miRNA in Serum of Patients with Prostate Cancer

Author(s): HAIMEI WANG, L. WANG, Z. WU, Q. LI, LIYUAN HUANG, F. JIANG, S. WANG*
Department of Urology, The Affiliated Huai’an Hospital of Xuzhou Medical University and The Second People’s Hospital of Huai’an, No.62, Huaihai Road(S.), Huai’an, Jiangsu 223002, 1Department of Urology, Yancheng Third People’s Hospital, 606 Xindu West Road, Yancheng, Jiangsu 224001, China

Correspondence Address:
S. WANG, Department of Urology, The Affiliated Huai’an Hospital of Xuzhou Medical University and The Second People’s Hospital of Huai’an, No.62, Huaihai Road(S.), Huai’an, Jiangsu 223002, China, E-mail: [email protected]


To explore the value of circulating micro ribonucleic acid expression in serum in the diagnosis of prostate cancer, this paper used The Cancer Genome Atlas database to download prostate cancer related datasets, analyze the expression of circulating micro ribonucleic acids in serum of prostate cancer tissues, screen differentially expressed micro ribonucleic acids, and find that micro ribonucleic acid-141 is significantly different. Then select 30 patients with prostate cancer in urology, and 20 patients with benign prostatic hyperplasia in the same period as research subject. The control group was 20 healthy subjects in the same period. The expression of serum micro ribonucleic acid-141 in control group, benign prostatic hyperplasia group and prostate cancer group was detected by fluorescent quantitative Polymerase Chain Reaction, and the content of total prostate specific antigen before treatment in prostate cancer group. The results showed that the expression level of serum micro ribonucleic acid-141 in the prostate cancer group was (11.2±8.1) times higher than that in the control group, which was significantly higher than that in the benign prostatic hyperplasia group (1.65+1.73) and the control group. The difference was statistically significant (p<0.05). The expression level of serum micro ribonucleic acid-141 in prostate cancer group was positively correlated with total prostate specific antigen level, correlation coefficient r=0.753, statistically significant (p<0.05). Serum micro ribonucleic acid-141 expression level is associated with Gleason score, clinical stages and occurrence of metastasis. With the increase of clinical stage, the expression level of micro ribonucleic acid increased. It is concluded that serum circulating micro ribonucleic acid-141 can be used as a molecular indicator for the diagnosis of prostate cancer.

PDF