Abstract
Exploring the Impacts of Mucin 1 Gene Expression on Apoptosis and Autophagy in Thyroid Cancer Cells via Adenosine-Monophosphate Activated-Protein Kinase-Mammalian Target of Rapamycin Signaling Pathway
Department of General Surgery, The First Affiliated Hospital of Xian Jiao Tong University, 1Department of Head and Neck Oncology, Shaanxi Provincial Tumor Hospital, Xi’an, Shaanxi Province 710061, China
Correspondence Address:
Wei Cheng, Department of Head and Neck Oncology, Shaanxi Provincial Tumor Hospital, Xi’an, Shaanxi Province 710061, China, E-mail: chengwei9264@163.com
Role of the Mucin 1 gene in thyroid cancer and its relationship with the adenosine-monophosphate activated-protein kinase-mammalian target of rapamycin signaling pathway were investigated in this work. The ACT-1 thyroid cancer cell lines were employed as the research models and enrolled into three groups based on treatment viz., a control group (no intervention), a small interfering ribonucleic acid-negative control group (transfection of negative control small interfering ribonucleic acid at 100 nM/l into ACT-1 thyroid cancer cells), and a Mucin 1-small interfering ribonucleic acid group (transfection of Mucin 1-small interfering ribonucleic acid at 100 nM/l into ACT-1 cells). The impacts of interfering with Mucin 1 gene on thyroid cancer cell proliferation, apoptosis, adenosine-monophosphate activated-protein kinase, mammalian target of rapamycin, and autophagy-related proteins (light chain 3I and light chain 3 II) were observed. The results indicated that the cell survival rate in Mucin 1-small interfering ribonucleic acid group (53.15±8.23) % was lower to that in control group (95.13±6.42) % (p<0.05), resulting a higher cell apoptosis rate of (15.79±1.36) %, which was (3.11±0.68) % in control group (p<0.05). p-Mammalian target of rapamycin in control group (0.13±0.02) was elevated based on that in Mucin 1-small interfering ribonucleic acid group (0.05±0.01) (p<0.05), while p-adenosine-monophosphate activated-protein kinase in control group (0.16±0.02) was downshifted in contrast to that in Mucin 1-small interfering ribonucleic acid group (0.46±0.03) (p<0.05). Light chain 3I/light chain 3II in control group was (0.28±0.02), being lower in comparison to that in Mucin 1-small interfering ribonucleic acid group (0.46±0.03) (p<0.05). Interference with Mucin 1 gene regulated the biological behaviors of thyroid cancer cells by modulating the adenosine-monophosphate activated-protein kinase-mammalian target of rapamycin signaling pathway.
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