Abstract

The present work describes about first order derivative spectroscopic approach for the estimation of carfilzomib as such and in parenteral formulation. The spectroscopic estimation was achieved by scanning the sample between 200 and 800 nm on shimadzu ultraviolet-1900 spectrophotometer. Further first order spectrums were obtained by taking derivatization of absorbance against wavelength. The developed method was validated as recommended in International Council for Harmonisation guideline. The linearity of the method was in the range of 6-30 μg/ml whereas correlation coefficient was 0.9991. The limits of detection and quantification were 1 μg/ml and 3 μg/ml respectively. Intra day and inter day system and method precision were determined and accuracy was between 99.03 %-100.59 %. The first order derivative spectroscopic estimation of carfilzomib is the subject of the proposed investigation. Different batches of samples were examined using this method. Furthermore, the method proved successful when applied to analyze a marketed formulation.