Gas Chromatography-Mass Spectrometry Analysis and Antihyperglycemic Property of Lindernia crustacea (L.) F. Muell.
Department of Zoology, Pharmacology and Bioinformatics Laboratory, Bodoland University, Kokrajhar, Assam 783370, India
A. Swargiary, Department of Zoology, Pharmacology and Bioinformatics Laboratory, Bodoland University, Kokrajhar, Assam 783370, India, E-mail: [email protected]
Lindernia crustacea (L.) F. Muell is a small herbaceous plant with several ethnomedicinal values. The present study investigated the phytochemical content and alpha-amylase and alpha-glucosidase inhibitory property of Lindernia crustacea. Methanolic crude extract of plant was obtained following the Soxhlet method. The crude extract was studied for alpha-amylase and alpha-glucosidase inhibitory activity. Phytochemical analysis was carried out using the gas chromatography-mass spectrometry technique. Furthermore, docking study was carried out with the phytocompounds to see the binding affinity with the enzymes. In silico drug-likeness and pharmacological properties were also carried out using Swiss absorption, distribution, metabolism and excretion, and absorption, distribution, metabolism, excretion and toxicity lab tools. The plant extracts showed concentration-dependent inhibition of enzyme activities with half-maximal inhibitory concentration values of 3.11 mg/ml and 548.9 μg/ml for alpha-amylase and alphaglucosidase, respectively. Gas chromatography-mass spectrometry study identified ten phytocompounds with molecular weights ranging from 264.4 to 561 g/mol. Docking study showed 1-(4-Hydroxybenzoyl)- 6,7-dimethoxyisoquinoline as the best binding compound with the enzymes. Phytocompounds identified from Lindernia crustacea were predicted to have substantial drug-likeness and absorption, distribution, metabolism, excretion and toxicity properties. The enzyme inhibition study and binding interactions of phytocompounds suggest promising alpha-amylase and alpha-glucosidase inhibitory activity of Lindernia crustacea. Therefore, the aerial part of Lindernia crustacea may be further investigated to know the exact mode of biological actions.Full-Text | PDF