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Heat Shock Protein 70 Inhibition Effect on Inflammatory Receptors via Nuclear Factor Kappa B on Melanoma Cell Lines, A-375 and C8161

Author(s): Yingying Wang, Lixin Wang, Linda Mohammadzadeh Boukani, N. Naghsh, S. Q. Abbas and Miaomiao Wang*
Department of Dermatology, Affiliated Hospital of Qingdao University, Qingdao, Shandong 266005, P. R. China, 1Department of Medicine, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz 15731, 2Department of Pharmacy, Shahid Sadoughi University of Medical Sciences, Yazd, Iran, 3Department of Medical Instruments Technology Engineering, Al-Turath University College, Al Mansour, Baghdad 10013, Iraq

Correspondence Address:
Miaomiao Wang, Department of Dermatology, Affiliated Hospital of Qingdao University, Qingdao, Shandong 266005, P. R. China, E-mail:

The aim of this study was evaluation of effect of heat shock protein 70 on toll-like receptor 4 and toll-like receptor 2 in melanoma cell line, A-375. The melanoma cell lines were stimulated in time course with heat shock protein 70 protein and incubation was performed. After 48 h, the cells were collected and total messenger ribonucleic acid was extracted and the expression of toll-like receptor 2 and toll-like receptor 4 was determined by quantitative reverse transcription polymerase chain reaction. The results showed that the appearance of toll-like receptor 2, toll-like receptor 4 in melanoma cell line, A-375 decreased in exposure to heat shock protein 70. The results showed the expression of nuclear factor kappa B in melanoma cell line. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide evaluation results of melanoma cell death were compared at different times and statistical analysis showed a significant difference (p<0.001). The role of heat shock protein 70 has been identified as a tumor suppressor in melanoma cancer. Heat shock protein 70 is a new treatment for melanoma in the near future. Heat shock protein 70 can be considered as a potential target in gene therapy for patients with melanoma cancer and the advantages of gene therapy by heat shock protein 70 in this type of cancer over chemotherapy can reduce cytotoxic effects and its low invasiveness.

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