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Abstract

High performance thin layer chromatographic estimation of lansoprazole and domperidone in tablets

Author(s): JV Susheel, M Lekha, TK Ravi
Department of Pharmaceutical Analysis, College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences, Coimbatore - 641 044, India

Correspondence Address:
T K Ravi Department of Pharmaceutical Analysis, College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences, Coimbatore - 641 044 India E-mail: [email protected]


A simple, fast, precise and accurate high performance thin layer chromatographic method has been developed for the simultaneous estimation of lansoprazole and domperidone in tablet formulations. This method allows the determination of 100-500 ng/spot of lansoprazole and 100-500 ng/spot of domperidone. The mobile phase composition was n-butanol:glacial acetic acid:water (9.3:0.25:0.5, v/v/v). Densitometric analysis of lansoprazole and domperidone was carried out in the absorbance mode at 288 nm. The R f values of lansoprazole and domperidone were found to be 0.78 and 0.21 respectively. The limit of detection for lansoprazole and domperidone were found to be 10 and 30 ng/spot, respectively. The limit of quantification for lansoprazole and domperidone were found to be 40 and 65 ng/spot, respectively. The amounts of drug present in the tablet and recovery studies were also carried out. The method was validated for precision, accuracy and reproducibility.

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