Abstract

A simple and sensitive high performance thin layer chromatography (HPTLC) method has been developed for the quantitative estimation of linezolid in its single component tablet formulations (600 mg). Linezolid was chromatographed on Silica Gel 60 F₂₅₄ TLC plate using methanol: benzene (2:8 v/v) as mobile phase. Linezolid showed Rf value 0.45 + 0.03 and scanned at 258 nm using a Camag TLC scanner 3. The method was validated in terms of linearity (200-1400 ng/spot), precision (intra-day variation, 0.75 to 1.26% and inter-day variation, 1.2 to 2.8%), accuracy (97.8 to 100.4%) and specificity. The limit of detection and limit of quantification for linezolid were found to be 16.7 ng/spot and 50.7 ng/spot, respectively. The developed method was successfully used for the assay of linezolid tablet formulations. The method was found to be simple, sensitive, specific, accurate and precise and can be used for the routine quality control testing of linezolid marketed formulations.