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Abstract

HPLC Analysis Of Withaferin A In Withania Somnifera (L.) Dunal

Author(s): Shashi Bala, R Govindarajan, A. K. S Rawat, Shanta Mehrotra

A reversed phase liquid chromatographic method for analysis and quantitative estimation of withaferin A in roots of Withania somnifera (L.) Dunal collected from different geographical zones, has been developed using a symmetry C18 column and a binary gradient profile. The various aspects of analysis such as extraction, efficiency, detection limits, reproducibility and peak purity were validated using photodiode array detector.

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