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Abstract

HPTLC method for the simultaneous estimation of emtricitabine and tenofovir in tablet dosage form

Author(s): Maithilee Joshi, AP Nikalje, M Shahed, M Dehghan
Y. B. Chavan College of Pharmacy, Dr. Rafiq Zakaria Campus, Rauza Bagh, Auranagabad-431 001, India

Correspondence Address:
A P Nikalje Y. B. Chavan College of Pharmacy, Dr. Rafiq Zakaria Campus, Rauza Bagh, Auranagabad-431 001 India [email protected]


A simple, precise, accurate and rapid high performance thin layer chromatographic method has been developed and validated for the estimation of emtricitabine and tenofovir simultaneously in combined dosage form. The stationary phase used was precoated silica gel 60F 254.The mobile phase used was a mixture of chloroform: methanol (9:1 v/v). The detection of spots was carried out at 265 nm. The method was validated in terms of linearity, accuracy, precision and specificity. The calibration curve was found to be linear between 200 to 1000 ng with regression coefficient of 0.9995.The proposed method can be successfully used to determine the drug content of marketed tablet formulation.

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