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Abstract

HPTLC method for the simultaneous estimation of valsartan and hydrochlorothiazide in tablet dosage form

Author(s): NJ Shah1, BN Suhagia2, RR Shah1, NM Patel1
1 Shri B. M. Shah College of Pharmaceutical Education & Research, Modasa-383 315, India 2 L. M. College of Pharmacy, Navrangpura, Ahmedabad-380 009, India

Correspondence Address:
N J Shah Shri B. M. Shah College of Pharmaceutical Education & Research, Modasa-383 315 India [email protected]


A simple, precise, accurate and rapid high performance thin layer chromatographic method has been developed and validated for the simultaneous estimation of valsartan and hydrochlorothiazide in combined dosage forms. The stationary phase used was precoated silica gel 60F 254 . The mobile phase used was a mixture of chloroform: methanol: toluene: glacial acetic acid (6:2:1:0.1 v/v/v/v). The detection of spots were carried out at 260 nm. The method was validated in terms of linearity, accuracy, precision and specificity. The calibration curve was found to be linear between 300 to 800 ng/spot for valsartan and 100 to 600 ng/spot for hydrochlorothiazide. The limit of detection and the limit of quantification for the valsartan were found to be 100 and 300 ng/spot respectively and for hydrochlorothiazide 30 and 100 ng/spot respectively. The proposed method can be successfully used to determine the drug content of marketed formulation.

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