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Abstract

Hydrolytic degradation profile and RP-HPLC estimation of cilostazol in tablet dosage form

Author(s): PK Basniwal1, PK Shrivastava2, Deepti Jain2
1LBS College of Pharmacy, Tilak Nagar, Jaipur-302 004, India 2School of Pharmaceutical Sciences, Rajiv Gandhi Proudyogiki Vishwavidyalaya (University of Technology of MP), Gandhinagar, Bhopal-462 036, India

Correspondence Address:
P K Basniwal LBS College of Pharmacy, Tilak Nagar, Jaipur-302 004 India E-mail: [email protected]


A simple, selective, precise and stability-indicating high-performance liquid-chromatographic method of analysis of cilostazol in pharmaceutical dosage form was developed and validated. The solvent system consisted of 10 mM phosphate buffer (pH 6.0):acetonitrile:methanol (20:40:40). Retention time of cilostazol in C18 column was 5.7 ± 0.1 min at the flow rate 1.3 ml/min. Cilostazol was detected at 248 nm at room temperature. The linear regression analysis data for the calibration plots showed good linear relationship with correlation coefficient value, r 2 =0.9998 in the concentration range 100-3200 ng/ml with slope 43.45 intercept 156.75. The method was validated for linearity, range, accuracy, precision and specificity. Cilostazol was determined in tablet dosage form in range of 99.58-100.67% with 0.4600 standard deviation. Stress studies were conducted in acid and alkali hydrolysis with gradual increasing concentration. Cilostazol was found to be stable in various concentrations of acidic and alkaline.

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