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Abstract

Icariin Regulates Cell Cycle Related Proteins and Apoptosis Related Proteins and Affects Cell Cycle and Apoptosis of Medulloblastoma Cells

Author(s): C. Xu, M. Xu, Yan Lu and X. Zhao*
Department of Pediatrics, Nantong First People's Hospital, Nantong, Jiangsu Province 226001, China

Correspondence Address:
X. Zhao, Department of Pediatrics, Nantong First People's Hospital, Nantong, Jiangsu Province 226001, China, E-mail: [email protected]


To investigate the effect of icariin on cell cycle and apoptosis of medulloblastoma cells and its mechanism. Medulloblastoma cell line D341 was cultured and divided into control group, low dose group, medium dose group and high dose group. The number and area of cell clones in low, medium and high dose groups were significantly lower than those in control group, and decreased with the increase of dose (p<0.05). The proportion of synthesis phase in the low, medium and high dose groups was significantly higher than that in the control group and increased with the increase of dose, while the proportion of growth 1 phase in the low, medium and high dose groups was significantly lower than that in the control group and decreased with the increase of dose (p<0.05). The protein expressions of cyclin A, cyclin-dependent kinase 2 and cyclin B1 in low, medium and high dose groups were significantly lower than those in control group and decreased with the increase of dose (p<0.05). The apoptosis rate of low, medium and high dose groups was significantly higher than that of control group and increased with the increase of dose (p<0.05). The expression of Bcl-2-associated X protein in low, medium and high dose groups was significantly lower than that in control group and decreased with the increase of dose. The expression of B-cell lymphoma 2, cleaved caspase-3, cleaved caspase-9 and cleaved poly (ADP-ribose) polymerase protein in low, medium and high dose groups was significantly higher than that in control group and increased with the increase of dose (p<0.05). Icariin can inhibit the proliferation and colony forming ability of medulloblastoma cells, induce cell arrest in synthesis phase by inhibiting the expression of cyclin A, cyclin-dependent kinase 2 and cyclin B1, and induce cell apoptosis by regulating the expression of B-cell lymphoma 2, cleaved caspase-3, cleaved caspase-9 and cleaved poly (ADP-ribose) polymerase protein.

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Citations : 53647

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