Abstract
In vitro Antihyperglycemic and Chelating Potential of Selected Ayurvedic Medicinal Plants
Unit of formation and Research in Sciences and Technology, University Norbert Zongo, Koudougou, 1Laboratory of Applied Biochemistry and Chemistry (LABIOCA), UFR/SVT, University Ouaga 1 Pr Joseph Ki-Zerbo, 09 BP 848 Ouagadougou 09, Burkina Faso, 2Division of Bioresource, Paper and Coatings Technology, School of Industrial Technology, 3School of Biological Sciences, Universiti Sains Malaysia, Penang, Malaysia, 4National Center of Scientific Research and Technology, Institute of Environment and Agricultural Research, Crop Production Department, 01 P.O. Box 476 Ouagadougou, Burkina Faso
Correspondence Address:
Laboratory of Applied Biochemistry and Chemistry (LABIOCA), UFR/SVT, University Ouaga 1 Pr Joseph Ki-Zerbo, 09 BP 848 Ouagadougou 09, Burkina Faso, E-mail: coulahmede@yahoo.fr
Seven medicinal plants used in folk medicine to relieve various diseases including diabetes, were investigated for antihyperglycemic activities. Two types of extracts for each plant were processed by decoction and by soaking in an aqueous-acetone mixture. The inhibitory activities were tested on α-amylase and α-glucosidase along with chelating activity. The aqueous-acetone extracts of Feretia apodanthera and Ozoroa insignis exerted strong inhibition on α-glucosidase (96.51±0.14 and 96.44±0.68 %, respectively) but lower inhibition on α-amylase (21.60±1.69 and 93.85±6.58 %, respectively) and displayed a weak chelating activity as compared to the respective standards acarbose and ethylenediaminetetraacetic acid. Subsequent column fractionation resulted in selectively strong inhibition of α-glucosidase. The inhibitory activities of α-amylase and α-glucosidase were weakly correlated (R=0,389) and both were inversely correlated with metal chelating activity (R=-0.731 and R=-0.809, respectively). Volatile compounds, 1-nonadecene and 1-heptadecene identified by GC-MS were related to the inhibitory activities of α-amylase and α-glucosidase enzymes. This potent inhibition of sugar degrading enzymes may help to manage the hyperglycemia occurring in type 2 diabetes.
