Abstract
Impacts of Anthocyanin on Apoptosis and Toll-Like Receptor 4/Cyclooxygenase 2/Nuclear Factor-Kappa B Signaling Pathway in Rats with Spinal Cord Ischemia-Reperfusion Injury
Department of Traditional Chinese Medicine, Huzhou Traditional Chinese Medicine Hospital Affiliated to Zhejiang Chinese Medical University, 1First Affiliated Hospital of Huzhou University, Huzhou, Zhejiang Province 310003, China
Correspondence Address:
Songhua Guo, First Affiliated Hospital of Huzhou University, Huzhou, Zhejiang Province 310003, China, E-mail: xueqiang2002@126.com
To investigate the impact of anthocyanin on apoptosis of spinal cord ischemia-reperfusion injury rats and its regulation on Toll-like receptor 4/cyclooxygenase 2/nuclear factor-kappa B signaling pathway. Sixty healthy Sprague–Dawley rats were randomly separated into sham operation group, model group, anthocyanin low (50 mg/kg), medium (100 mg/kg), high (150 mg/kg) concentration groups, anthocyanin (150 mg/kg)+TAK-242 (10 mg/kg) group, 10 per group, administered at 72 h before surgery. After the experiment, the behavioral score after spinal cord injury was performed; blood was collected to separate serum, and the contents of interleukin-6, interleukin-1 beta and tumor necrosis factor alpha were measured; the rats were sacrificed to separate spinal cord tissue, and terminal deoxynucleotidyl transferase dUTP nick-end labeling staining was used to detect cell apoptosis, the expression of B-cell lymphoma protein 2-associated X was measured by immunohistochemistry, the expression of toll-like receptor 4, cyclooxygenase 2, p-nuclear factor-kappa B and nuclear factor-kappa B proteins was measured by Western blot. Compared with the sham operation group, the behavioral score and B-cell lymphoma protein 2-associated X expression in the model group were obviously decreased (p<0.05); the apoptosis index, serum interleukin-6, interleukin-1 beta, tumor necrosis factor alpha contents, B-cell lymphoma protein expression, toll-like receptor 4, cyclooxygenase 2 protein expression and p-nuclear factor-kappa B/nuclear factor-kappa B in spinal cord tissue were obviously increased (p<0.05). Compared with the model group, the behavioral scores and B-cell lymphoma protein 2-associated X expression in the anthocyanin low, medium and high concentration groups were obviously increased (p<0.05); the apoptosis index, serum interleukin-6, interleukin-1 beta, tumor necrosis factor alpha contents, B-cell lymphoma protein 2 expression, toll-like receptor 4, cyclooxygenase 2 protein expression and p-nuclear factor-kappa B/nuclear factor-kappa B in spinal cord tissue were gradually decreased (p<0.05). Compared with the anthocyanin high concentration group, the behavioral score and B-cell lymphoma protein 2-associated X expression in the anthocyanin+TAK-242 group were obviously increased (p<0.05); the apoptosis index, serum interleukin-6, interleukin-1 beta, tumor necrosis factor alpha contents, B-cell lymphoma protein 2 expression, toll-like receptor 4, cyclooxygenase 2 protein expression and p-nuclear factor-kappa B/nuclear factor-kappa B in spinal cord tissue were obviously decreased (p<0.05). Anthocyanin can ameliorate cell apoptosis in spinal cord ischemia-reperfusion injury rats by inhibiting the expression of toll-like receptor 4/cyclooxygenase 2/nuclear factor-kappa B signaling pathway.
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