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In vitro evaluation of Eclipta alba against serogroups of Leptospira interrogans

Author(s): N Prabhu1, Joseph Pushpa Innocent2, P Chinnaswamy3, K Natarajaseenivasan4, Lakshmi Sarayu2
1Postgraduate and Research Department of Microbiology, Dr. NGP Arts and Science College, Coimbatore-641 035, India 2Division of Microbiology, Rajah Muthiah Medical College and Hospital, Annamalai University, Chidambaram, India 3Institute of Laboratory Medicine, Kovai Medical Center and Hospital, Coimbatore-641 014, India 4Department of Microbiology, School of Life Sciences, Bharathidasan University, Tiruchirapalli-620 025, India

Correspondence Address:
N Prabhu Postgraduate and Research Department of Microbiology, Dr. NGP Arts and Science College, Coimbatore-641 035 India [email protected]

Leptospirosis is now acknowledged as the most widespread zoonoses in the world. Hundreds of cases occur in India every year accounting for considerable morbidity and sizable mortality. Several studies have delineated the epidemiology, pathology and variable clinical features of this condition. The present study comprises the importance and utilization of traditional based medicines to overcome the adverse reaction by conventional drugs and standardize the technology. The antileptospiral activity of Eclipta alba L. was well studied by both tube dilution and micro dilution techniques and the result showed better inhibitory action against various serogroups of Leptospira interrogans. L. australis, L. autumnalis and L. grippotyphosa are inhibited by both water and ethanol extract by tube dilution technique. The MIC level observed are 50 µg and 100 µg respectively. Similarly acetone extract, Icterohaemorrhagiae was responded to 200 µg/ml as MIC whereas in petroleum ether extract, no inhibition was observed. In the case of micro dilution technique, the entire inhibition rates are supported to the tube dilution technique. It showed that the micro dilution technique is the best method where we obtained the results within 30 minutes; at the same time tube dilution technique takes minimum of 7 days to provide the result.

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