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Abstract

Inhibition of ERCC5 Attenuates Proliferation and Invasion while Promotes Apoptosis in Ovarian Cancer

Author(s): LI JIANG, XINHUA BU*, HUA QIAN, DONGLAN YUAN, SHAOJUN WU AND YINLING ZHAO
Department of Obstetrics and Gynecology, Jiangsu Taizhou People’s Hospital, the Fifth Affiliated Hospital of Nantong University, Taizhou, Jiangsu 225300, P. R. China

Correspondence Address:
Department of Obstetrics and Gynecology, Jiangsu Taizhou People’s Hospital, the Fifth Affiliated Hospital of Nantong University, Taizhou, Jiangsu 225300, P. R. China, E-mail: [email protected]

The present investigation dealt with differential expression of ERCC5 in different ovarian cancer cells and the potential mechanism involved. By detecting the expression in both SKOV3 and Caov4 cell lines, it was found that the expression of ERCC5 in SKOV3 was significantly higher than that in Caov4. An SKOV3 cell line with low ERCC5 expression by ribonucleic acid interference and Caov4 cell line with high ERCC5 expression by transient transfection was constructed to compare the effect of ERCC5 abnormal expression on the biological behaviour of ovarian cancer cells. The results showed that high expression of ERCC5 could significantly promote proliferation and invasion of ovarian cancer but inhibit apoptosis, while low expression of ERCC5 could significantly promote apoptosis and inhibit proliferation and invasion. The effect of ERCC5 abnormal expression on potential regulation pathways was studied using Western blot. It was found that ERCC5 abnormal expression could regulate the Wnt pathway. It was demonstrated that inhibition of ERCC5 expression could attenuate proliferation and invasion while promote apoptosis of ovarian cancer cells by suppressing the Wnt pathway

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