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Abstract

Inhibition of miR-5571-3p Attenuates Proliferation and Invasion in Rheumatoid Arthritis Fibroblast-like Synoviocytes by Upregulating OAZ3

Author(s): J. LIN*, B. X. LIU AND H. M. ZOU
Department of Rheumatology and Immunology, Taizhou People’s Hospital, No. 366 Taihu Road, Taizhou, Jiangsu 225300, China

Correspondence Address:
Department of Rheumatology and Immunology, Taizhou People’s Hospital, No. 366 Taihu Road, Taizhou, Jiangsu 225300, China, E-mail: m16531755539_1@163.com


The aim of the present study was to explore the role and potential mechanism of miR-5571-3p in rheumatoid arthritis. In this study, miR-5571-3p level in rheumatoid arthritis tissues and fibroblast-like synoviocytes was detected using qRT-PCR analysis. Cell Counting Kit-8 assay was performed to determine proliferation of fibroblast-like synoviocyte. Transwell assay was conducted to examine the invasion of fibroblast-like synoviocytes, and western blot analysis was used to examine the ornithine decarboxylase antizyme 3 protein expression. Results showed that miR-5571-3p expression was elevated in vivo and in vitro in rheumatoid arthritis. Inhibition of miR-5571-3p suppressed rheumatoid arthritis fibroblastlike synoviocyte proliferation and invasion. ornithine decarboxylase antizyme 3 siRNA could partially abolish the suppressor effect of miR-5571-3p inhibitor in cell proliferation and invasion in fibroblastlike synoviocytes. In conclusion, miR-5571-3p and ornithine decarboxylase antizyme 3 played important regulatory roles in proliferation and invasion of rheumatoid arthritis fibroblast-like synoviocytes, miR5571-3p/ornithine decarboxylase antizyme 3 axis could serve as a new target for therapy of rheumatoid arthritis.

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