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Abstract

Investigating the Impact of Astragalus Polysaccharide on Angiogenesis in Human Adipose-Derived Stem Cells

Author(s): Wen Ke, Zhang Jun, Shi Jingjun, Chen Xu and Huang Jinlong*
Department of Plastic Surgery, Affiliated Hospital of Nanjing University of Chinese Medicine, 1Department of Breast and Endocrine Surgery, Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing, Jiangsu Province 210000, China

Correspondence Address:
Huang Jinlong, Department of Breast and Endocrine Surgery, Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing, Jiangsu Province 210000, China, E-mail: hjlsurgeon@163.com


This study aims to investigate the influence of Astragalus polysaccharides on angiogenesis in human adiposederived stem cells. Three experimental groups were established; control group (group A), a low-dose Astragalus polysaccharides intervention group (group B), and a high-dose Astragalus polysaccharides intervention group (group C). Group A comprised untreated human adipose-derived stem cells cultured under standard conditions. Group B received an Astragalus polysaccharides concentration of 80 μg/ml, while group C was treated with 160 μg/ml of Astragalus polysaccharides. Cell proliferation, migration, and angiogenic potential were assessed using cell counting kit-8 assay, Western blot analysis, quantitative polymerase chain reaction, and Transwell migration assays. Relative to group A, group B exhibited enhanced cell migration and proliferation, with further increases observed in group C. Angiopoietin-1 and vascular endothelial growth factor levels were elevated in group B compared to group A, with an additional increase in group C. Conversely, the expression levels of pro-apoptotic proteins Bcl-2-associated X protein and caspase-3 were decreased in group B relative to group A, and further reduced in group C. Hypoxia-inducible factor 1 and nuclear factor kappa B expression were diminished in group B compared to group A, with a more pronounced reduction in group C. Astragalus polysaccharides modulate angiogenesis in human adipose-derived stem cells by downregulating nuclear factor kappa B expression, thereby inhibiting hypoxia-inducible factor 1 and nuclear factor kappa B pathways. This modulation facilitates the proliferation and angiogenic activity of adipose-derived stem cells, suggesting a promising therapeutic potential of Astragalus polysaccharides in regenerative medicine and tissue engineering.

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