Abstract

To explore the effect and possible mechanism of iridoid glucosides from Boschniakia rossica on ovarian cancer cell proliferation and metastasis. Human ovarian cancer cells (OVCAR3) were treated with different concentrations of iridoid glucosides from Boschniakia rossica. Also, human ovarian cancer cells were transfected with plasmid cloning deoxyribonucleic acid, plasmid cloning deoxyribonucleic acid circular-homeodomain-interacting protein kinase 3, microRNA-NC+plasmid cloning deoxyribonucleic acid-circular-homeodomain-interacting protein kinase 3 and microRNA-495-3p mimics+plasmid cloning deoxyribonucleic acid-circular-homeodomain-interacting protein kinase 3, followed by treated with high-dose of iridoid glucosides from Boschniakia rossica. Cell functions were tested by colony formation, transwell and 3-(4, 5-dimethylthiazol-2-yl)-2, 5 diphenyl tetrazolium bromide assays. Gene levels were examined using quantitative reverse transcription polymerase chain reaction and Western blot method. Ribonuclic acid interaction was verified using dual-luciferase reporter assay. Iridoid glucosides from Boschniakia rossica could reduce OVCAR3 cell proliferation and metastasis. Moreover, iridoid glucosides from Boschniakia rossica decreased circular-homeodomain-interacting protein kinase 3 expression and increased microRNA-495-3p expression. Plasmid cloning deoxyribonucleic acid-circular-homeodomain-interacting protein kinase 3 reversed iridoid glucosides from Boschniakia rossica-mediated the inhibition on OVCAR3 cell functions. Circular-homeodomain-interacting protein kinase 3 targeted microRNA-495-3p. Also, microRNA-495-3p mimics abolished the effects of plasmid cloning deoxyribonucleic acid-circular-homeodomain-interacting protein kinase 3 on iridoid glucosides from Boschniakia rossica-mediated cell functions. Iridoid glucosides from Boschniakia rossica inhibited ovarian cancer cell proliferation and metastasis by circular-homeodomain-interacting protein kinase 3/microRNA-495-3p.