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Abstract

Isolation and Identification of Bacteriocin Producing Microbial Strains from Rhizosphere Soil

Author(s): Vahid, Sarita Mallik* and Muskan Tyagi
School of Biological and Life Sciences, Galgotias University, Greater Noida, Uttar Pradesh 203201, India

Correspondence Address:
Sarita Mallik, School of Biological and Life Sciences, Galgotias University, Greater Noida, Uttar Pradesh 203201, India, E-mail: sarita81@gmail.com


Emergence and spread of antibiotics resistance among bacteria poses a serious concern for human health and disease therapy. In addition to this, novel pathogens are also surfacing rapidly. Discovery and development of newer, potent antimicrobial and therapeutic agents is constantly required. Soil is perplexing at different climatic conditions thereby providing variable source for the production of antibiotic producing microorganisms. Bacteriocin is a proteinaceous substance produced by bacteria which inhibits the growth of closely or exactly related bacterial strains. It is natural, safe secondary metabolite and its development and need for better antibiotic spectrum is always in high demand. In the present investigation, the bacteriocin producing microorganisms were isolated from the local rhizospheric soil samples. The isolates were subject to antibacterial susceptibility test against the common pathogenic bacteria i.e., Pseudomonas, Aeromonas, Bacillus and Escherichia coli by agar well diffusion method. The prominent zone of inhibition shown by isolate strains was recorded. Out of the 11 isolates, 5 isolated bacterial strains displayed significant inhibitory activity against the pathogenic bacteria. For further identification, biochemical tests were performed for these strains. These bacterial strains were identified with high similarity to Streptococcus sp., Enterococcus and Gemella berger. This investigation indicates that these bacteria have great potential for the production of antimicrobial property and this can be further characterized and utilized to control the growth of microbes in the future.

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