All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Abstract

Kaempferol Promotes Apoptosis and Inhibited Autophagy in A549 Cells via MicroRNA-199/Mammalian Target of Rapamycin Axis

Author(s): Junjie Wang and Bo Han*
Department of Cardiothoracic Vascular Surgery, The Third Affiliated Hospital of Inner Mongolia Medical University (Inner Mongolia Baotou Steel Hospital), Baotou, Inner Mongolia 010107, China

Correspondence Address:
Bo Han, Department of Cardiothoracic Vascular Surgery, The Third Affiliated Hospital of Inner Mongolia Medical University (Inner Mongolia Baotou Steel Hospital), Baotou, Inner Mongolia 010107, China, E-mail: [email protected]


To explore the function of kaempferol on the lung cancer cell A549 and the microRNA-199 variation is the objective of the study. The cell proliferation was detected by cell counting kit-8. The expression of mammalian target of rapamycin, phosphorylated mammalian target of rapamycin, light chain 3-?/?, beclin-1, B-cell lymphoma 2 and B-cell lymphoma 2-associated X protein was showed by Western blot. The microRNA-199 expression was determined by quantitative polymerase chain reaction. The adenocarcinomic human alveolar basal epithelial cells, A549 proliferation was gradually low with the concentration of kaempferol rising. Kaempferol could inhibit the microRNA-199, phosphorylated mammalian target of rapamycin, light chain 3-?/?, beclin-1 and B-cell lymphoma 2-associated X protein expression and promoted B-cell lymphoma 2 expressions. When microRNA-199 inhibited the expression of phosphorylated mammalian target of rapamycin, light chain 3-?/? and beclin-1 in the kaempferol+microRNA-199 inhibitor group was lower than blank group and kaempferol added only group. In the kaempferol+microRNA-199 inhibitor group, the B-cell lymphoma 2 was lower than the blank group and kaempferol added only group. When the mammalian target of rapamycin was inhibited the expression of light chain 3-?/? and beclin-1 was low. The B-cell lymphoma 2 was low and the B-cell lymphoma 2-associated X protein was high in the two groups. The kaempferol promoted the apoptosis and inhibited autophagy in A549 cells via microRNA/mammalian target of rapamycin axis.

Full-Text | PDF

 
 
Google scholar citation report
Citations : 53647

Indian Journal of Pharmaceutical Sciences received 53647 citations as per google scholar report