All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Abstract

Laparoscopic Cholecystectomy Intervention in Rats with Severe Acute Pancreatitis Delays Apoptosis of Myocardial Cells

Author(s): X. Ye, M. Wang, X. Fang, C. Tu, L. Wang*
Department of ICU, Shanghai Yangpu District Shidong Hospital, Shanghai, 200438, China

Correspondence Address:
Department of ICU, Shanghai Yangpu District Shidong Hospital, Shanghai, 200438, China, Email: [email protected]

In order to study the mechanism of laparoscopic cholecystectomy (LC) intervention in macrophages of rats with severe acute pancreatitis to delay apoptosis of cardiac myocytes, Wistar rats were chosen and divided into 3 groups. In addition, the related indices and protein expression in rats were observed at 2, 8, 16 and 24 h for each component and caspase-3 and bcl-2 levels in myocardium are detected to study the effect of laparoscopic cholecystectomy intervention. Results indicated that there were no obvious symptoms in control rats, while the rats in intervention group have the same symptoms as the experimental group. White blood cells of the 3 groups of rats, when compared it was found that the white blood cells in the experimental group decrease, followed by the intervention group but there is no significant change in the control group. Morphological examination of myocardial tissue from the 3 groups of rats indicated that there was no significant change in myocardial cells in the control group, while piles of blood cells were found between myocardial cells in the experimental group with obvious nuclear deformation. There were patches of blood between myocardial cells in the intervention group, without any significant changes in nucleus. Among the changes in the content of tumor necrosis factor-α and apoptosis of myocardial tissue, it is found that the tumor necrosis factor-α content and apoptotic phenomena were most significant in the experimental group and there was a significant correlation between the experimental group and the control group. After laparoscopic cholecystectomy, it was found that the content of tumor necrosis factor-α in myocardial tissue decreased and apoptotic phenomena were alleviated, which was significantly different from the experimental group (P < 0.01). Western blot results indicated that expression of caspase-3 and bcl-2 in myocardial tissue were the highest in the experimental group, followed by the intervention group and the lowest in the control group. It was also found that the change of tumor necrosis factor-α was synchronous and positively correlated with the change of apoptotic index of myocardial cells. Therefore, it could be concluded that laparoscopic cholecystectomy could be used to intervene in severe acute pancreatitis to delay the apoptosis of myocardial cells in rats..

PDF