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Abstract

Long Non-Coding RNA Taurine Upregulated Gene 1 Affects Cell Apoptosis and Cell Cycle through Downregulating Cell Division Control Protein 42 Homolog in Osteoblasts

Author(s): YITING CAO, Y. WU, Q. GU, FANG FANG AND QIAN JIANG*
Department of Pediatric Dentistry, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, 1College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai 200240, 2Shanghai Xuhui District Dental Center, Shanghai 200031, 3Departments of Prosthodontics, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 4Departments of Oral Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China

Correspondence Address:
QIAN JIANG, Departments of Oral Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China, E-mail: [email protected]


To explore the regulation and potential mechanism of long non-coding ribonucleic acid taurine upregulated gene 1 on the apoptosis and cell cycle of osteoblasts is the main purpose. The overexpression plasmid and small interfering ribonucleic acid were used to regulate the expression of taurine upregulated gene 1 in osteoblasts. The expression of taurine upregulated gene 1 was detected by quantitative reverse transcription polymerase chain reaction and the effect of taurine upregulated gene 1 on the apoptosis and cell cycle of osteoblasts were detected by flow cytometry, quantitative reverse transcription polymerase chain reaction and western blot analyses were used to detect the regulation of taurine upregulated gene 1 on cell division control protein 42 homolog and combined with functional recovery experiments to verify that taurine upregulated gene 1 regulated the apoptosis and cell cycle of osteoblasts through cell division control protein 42 homolog. Silencing taurine upregulated gene 1 promoted the apoptosis of osteoblasts and blocked osteoblasts from entering the growth 2 phase/mitotic phase. Overexpression of taurine upregulated gene 1 had no significant effect on the apoptosis and cell cycle of osteoblasts. Silencing taurine upregulated gene 1 inhibited the expression of cell division control protein 42 homolog and overexpression of taurine upregulated gene 1 promoted the expression of cell division control protein 42 homolog. In addition, compared with the taurine upregulated gene 1 overexpression group, the apoptosis of osteoblasts in the taurine upregulated gene 1 overexpression+cell division control protein 42 homolog antagonist group increased significantly. Compared with the taurine upregulated gene 1 silencing group, the apoptosis of osteoblasts in the taurine upregulated gene 1 silencing+cell division control protein 42 homolog agonist group was significantly reduced and the number of osteoblasts entering the growth 2 phase/mitotic phase was significantly increased. Silencing taurine upregulated gene 1 promotes the apoptosis of osteoblasts and prevented osteoblasts from entering growth 2 phase/mitotic phase by downregulating the expression of cell division control protein 42 homolog. The normal expression level of taurine upregulated gene 1 maintains the normal apoptosis and cycle of osteoblasts by stabilizing the expression of cell division control protein 42 homolog.

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