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Abstract

Matrix solid-phase dispersion extraction and quantification of alpinetin in amomum seed using validated HPLC and HPTLC methods

Author(s): M Singh1, YT Kamal1, MA Khan2, Rabea Parveen3, SH Ansari1, S Ahmad1
1Department of Pharmacognosy and Phytochemistry, Hamdard University, Hamdard Nagar, New Delhi-110 062, India 2Department of Pharmacology, Hamdard University, Hamdard Nagar, New Delhi-110 062, India 3Department of Pharmaceutics, Faculty of Pharmacy, Hamdard University, Hamdard Nagar, New Delhi-110 062, India

Correspondence Address:
S Ahmad Department of Pharmacognosy and Phytochemistry, Hamdard University, Hamdard Nagar, New Delhi-110 062 India E-mail: [email protected]


Alpinetin is a flavonoidal constituent of seeds of Amomum subulatum Roxb., recently reported to possess vasorelaxant and antiHIV activities. Simple, accurate and precise HPLC and HPTLC methods were developed for the analysis of alpinetin in A. subulatum seed extracts and extraction technique was optimized to get maximum yield using conventional, ultrasonic and matrix solid phase dispersion extraction. HPLC was performed on a C18 column with methanol and water (70:30, v/v) as mobile phase at a flow rate of 1.0 ml/min whereas HPTLC on silica aluminum sheet (60F 254 ) using toluene, dichloromethane and ethyl acetate as solvent system. A sharp peak was obtained for alpinetin at a retention time (R t ) of 5.7 min by HPLC and retardation factor (R f ) of 0.48 by HPTLC. Both methods were validated as per the ICH guidelines and the content of alpinetin was estimated in different extracts. Matrix solid phase dispersion technique was found most suitable for extracting alpinetin as compared to other techniques. Validation data are indicative of good precision and accuracy and proved the reliability of the methods.

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