Micro RNA-483 Elevates Nucleus Pulposus Cell Proliferation and Matrix Degradation in Intervertebral Disc Degeneration
The First Clinical Medical College of Zhejiang Chinese Medical University, No. 546, Binwen Road, Hangzhou, Zhejiang 310053, 1Department of Chronic Wound Diagnosis and Treatment Center, Hangzhou Geriatric Hospital, No. 469, Shen Ban Road, Hangzhou, Zhejiang 310022, China
Department of Chronic Wound Diagnosis and Treatment Center, Hangzhou Geriatric Hospital, No. 469, Shen Ban Road, Hangzhou, Zhejiang 310022, China, E-mail: firstname.lastname@example.org
This study was to evaluate the function of miR-483 in the development of intervertebral disc degeneration in order to find a potential novel therapeutic target for intervertebral disc degeneration. MiR-483 expression was determined in nucleus pulposus tissue, cells from patients with intervertebral disc degeneration and controls using real-time PCR. Western blotting was performed to determine anticollagen II, matrix metalloproteinase-2, matrix metallopeptidase-9, cyclin A, cyclin D1 and glyceraldehyde-3-phosphate dehydrogenase expression. Nucleus pulposus cell growth was detected in a MTT assay. Cell cycle was measured following flow cytometric detection. The expression level of miR-483 was downregulated significantly in intervertebral disc degeneration nucleus pulposus samples. Besides, miR-483 expression was negatively associated with the Pfirrmann grade. Furthermore, overexpression of miR-483 could promote nucleus pulposus cell growth, enhance the anticollagen II expression as well as inhibit the matrix metalloproteinase-2 and 9 expression. In addition, miR-483 caused an increase in the proportion of S-phase of nucleus pulposus cells. Present study revealed a novel miRNA in the progression of intervertebral disc degeneration, which could serve as a potential therapeutic approach for intervertebral disc degeneration.