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Abstract

MicroRNA-16-5p inhibits the Proliferation and Invasion of Glioma Cells by the Activation of p53/BAX Signal Pathway

Author(s): SHAO CAI HAO, Z. LIU, J. FENG, HUI MA* AND HE CHUN XIA*
Department of Neurosurgery, The General Hospital of Ningxia Medical University, 804 Shengli Street, Yinchuan, Ningxia 750004, China

Correspondence Address:
Department of Neurosurgery, The General Hospital of Ningxia Medical University, 804 Shengli Street, Yinchuan, Ningxia 750004, China, E-mail: [email protected]

Glioma was one of the most common malignant brain tumors. The effect of routine clinical treatment of glioma was not ideal, and the molecular mechanism of the occurrence and development of the disease was still unclear. In this paper, the mechanism of MicroRNA-16-5p in the occurrence and development of glioma was studied by the cell experiment in vitro. Through the analysis of the The Cancer Genome Atlas database and clinical tissue samples, the expression of MicroRNA-16-5p in glioma was detected. The inhibitory effect of MicroRNA-16-5p on the proliferation, invasion, cell cycle and apoptosis of glioma cells was evaluated by the cell proliferation, invasion and flow cytometry. The relationship between the expression of p53, Bcl-2 Associated X-protein and MicroRNA-16-5p was studied by dual-luciferase gene assay, Quantitative Reverse transcription- polymerase chain reaction, and Western blotting. Compared with the normal tissues, the expression of MicroRNA-16-5precursor was low in glioma tissues, which was consistent with that in the Uppsala87 and Uppsala251 cell lines. The over expression of MicroRNA-16-5p can inhibit the growth and invasion of the glioma cell line, promote the apoptosis and delay the cell cycle. The results of Quantitative Reverse transcription- polymerase chain reaction and Western blotting showed that the target gene of MicroRNA-16-5p was p53, and the over expression of MicroRNA-16-5p could promote the expression of p53 and Bcl-2 Associated X-protein, which was positively correlated. MicroRNA-16-5p was a tumor suppressor in the progression of glioma. We have demonstrated the MicroRNA-873-5p-p53/ Bcl-2 Associated X-protein regulatory pathway in the development of glioma, which provides a theoretical basis for the development of the alternative strategies for the clinical treatment of glioma.

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