Abstract

The aim of this study was to research the association between lysyl oxidase-like 1-antisense 1 and microRNA-761, and explore the molecular mechanisms governing the growth and programmed cell death in ovarian cancer cells. To identify the targeting association between long noncoding RNA lysyl oxidase-like 1-antisense 1 and microRNA-761, the LncBase Predicted v.2 and dual luciferase report examination were employed. The 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide assay and flow cytometry were used to examine the apoptosis and proliferation of ovarian cancer cells, respectively. Western blot was performed to test the levels of cyclin D1, B-cell lymphoma-2, and Bcl-2-associated X protein expressions. SKOV-3 cells exhibited a notable increase in the expression of lysyl oxidase-like 1-antisense 1 compared to normal ovarian epithelial cells HOSE, while displaying a significant reduce in the expression of microRNA-761 than normal HOSE cells. The transfection of microRNA-761 caused a significant reduce in the relative luciferase activity of lysyl oxidase-like 1-antisense 1-WT. Suppression of lysyl oxidase-like 1-antisense 1 and upregulation of microRNA-761 resulted in a substantial reduce in the optical density value and horizontal of cyclin D1 and B-cell lymphoma-2 albumen expression in SKOV-3 cells after 24, 48, and 72 h. Additionally, it led to a raise in the rate of apoptosis and the expression level of Bcl-2-associated X protein albumen in SKOV-3 cells. Co-transfection of si-lysyl oxidase-like 1-antisense 1 and anti-microRNA-761 resulted in a significant decrease in microRNA-761 expression, apoptosis rate, and Bcl-2-associated X protein levels in SKOV-3 cells. Additionally, it led to an increase in optical density value and cyclin D1 horizontals in SKOV-3 cells at 24, 48, and 72 h. In conclusion, the elevated expression of long noncoding RNA lysyl oxidase-like 1-antisense 1 in ovarian cancer cells is associated with its close interaction with the targeted negative regulation of microRNA-761 expression, and knocking down long noncoding RNA lysyl oxidase-like 1-antisense 1 expression can enhance the apoptosis of ovarian cancer cells.