Neochamaejasmin A Induces Apoptosis in Human Osteosarcoma MG-63 Cells via Mitochondrial Dysfunction and Phosphatidylinositol 3 Kinase/Protein Kinase B/Glycogen Synthase Kinase-3-Beta Signaling Pathway
Department of Orthopedics, Wuhan Fourth Hospital, Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology, Hanzheng, Qiaokou, Wuhan 430033, China
Li Yan, Department of Orthopedics, Wuhan Fourth Hospital, Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology, Hanzheng, Qiaokou, Wuhan 430033, China, E-mail: firstname.lastname@example.org
Neochamaejasmin A is an individual compound isolated from Stellera chamaejasme and exerts antitumor effects on several types of human cancer cells. However, the effect of neochamaejasmin A on human osteosarcoma cells has not yet been investigated. The main aim was to detect the in vitro antitumor effect of neochamaejasmin A on human osteosarcoma MG-63 cells. MG-63 cells were treated with different concentrations of neochamaejasmin A (20, 40, 80 μg/ml). Cell viability was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Cell apoptosis was detected through cell morphology observation and Annexin V/propidium iodide staining. Mitochondrial membrane potential was measured by rhodamine 123 staining. The expressions of apoptosis-related proteins and phosphatidylinositol 3 kinase/protein kinase B signaling proteins were analyzed by western blotting. The results showed that neochamaejasmin A significantly inhibited proliferation and induced apoptosis of MG- 63 cells. Neochamaejasmin A triggered the disorder of mitochondrial membrane potential, increased the expression of Bcl-2-associated X protein and cleaved-caspase 3, decreased expression of B-cell lymphoma 2, and induced apoptosis through the inactivation of phosphatidylinositol 3 kinase/protein kinase B/glycogen synthase kinase-3-beta signaling pathway. These new findings may indicate that neochamaejasmin A has potential to be chosen as a candidate for osteosarcoma chemotherapy.