Abstract
Phytochemical Analysis and In Vitro Synergistic Anti-Bacterial Effect of Methanolic Extracts of Phyllanthus Fruits against Human Pathogens
Department of Applied Microbiology and Biochemistry, Sri Padmavati Mahila Visvavidyalayam, Tirupati, Andhra Pradesh 517502, 1Department of Biotechnology, Vikrama Simhapuri University, Nellore, Andhra Pradesh 524320, 2Department of Botany, Sri Venkateswara University, Tirupati, Andhra Pradesh 517502, India
Correspondence Address:
Suvarnalathadevi Potireddy, Department of Applied Microbiology and Biochemistry, Sri Padmavati Mahila Visvavidyalayam, Tirupati, Andhra Pradesh 517502, India, E-mail: drsuvarnaspmvv@gmail.com
The present work focused at antibacterial activity of three different phyllanthus species; phyllanthus acidus, phyllanthus emblica and phyllanthus niruri. The antibacterial activities of methanolic extracts of fruits were tested in vitro against human pathogenic bacteria such as Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumonia and Salmonella typhi. Ciprofloxacin (0.02 mg/ml), Gentamycin (0.02 mg/ml), and Tetracycline (0.02 mg/ml) antibiotics were used as standards by disc diffusion method. The synergistic activity of the three Phyllanthus species was investigated. The study found that at 100 g/ml, the methanolic extract of Phyllanthus emblica had the strongest action against all of the species, whereas Phyllanthus acidus and Phyllanthus niruri had the lowest activity .The synergistic activity of all the extracts showed potent activity. The Fourier-transform infrared spectroscopy and Ultraviolet-Visible spectrum revealed the existence of functional groups of carboxyl, carbonyl, and aromatic groups.
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