Phytochemical Analysis, Tuberculosis Effect, Purification and Characterization of Aerial Parts of Avicennia alba
Department of Chemical Engineering, Centre for Biotechnology, Andhra University College of Engineering, Andhra University, Visakhapatnam, Andhra Pradesh 530003, India
R A Zerubabel Michael, Department of Chemical Engineering, Centre for Biotechnology, Andhra University College of Engineering, Andhra University, Visakhapatnam, Andhra Pradesh 530003, India, E-mail: email@example.com
The present study deals with phytochemical, pharmacological (In vitro antioxidant), purification and characterization studies of aerial parts of Avicennia alba. The phytochemical analysis for qualitative and quantitative investigation revealed the presence of more bioactive compounds in methanolic extract than hexane and chloroform extracts of Avicennia alba. The methanolic extract showed better activity on 2,2-diphenyl-1-picrylhydrazyl free radical than remaining free radical tests i.e hydrogen peroxide and hydroxyl radical. The methanolic extract was potent in inhibiting the free radicals upon comparison with standard ascorbic acid. The tuberculosis effect of the plant was not up to the mark when compared with standard control. The methanolic plant extract was subjected to column chromatography and three compounds were isolated. The isolated compounds were characterized by ultraviolet, fourier transform infrared spectroscopy, proton nuclear magnetic resonance, carbon nuclear magnetic resonance and mass spectral data studies and were identified as stigmasterol, beta-sitosterol and lupeol. The work carried out was distinctive as the source of plant was taken from coringa mangrove, Kakinada, first of its kind and the tuberculosis effect was also reported for the first time, though it was not up to the mark the work reported inspires the researcher to enlighten and save their time. The natural compounds selected were reported previously but an in depth characterization was reported for a thorough look.