Polyphenol Profile, Antioxidant and Hypoglycemic Activity of Acalypha hispida Leaf Extract
Department of Anatomy, Physiology, and Pharmacology, Faculty of Veterinary Medicine, IPB University (Bogor Agricultural University), Campus Dramaga, Bogor 16680, Indonesia
Department of Anatomy, Physiology, and Pharmacology, Faculty of Veterinary Medicine, IPB University (Bogor Agricultural University), Campus Dramaga, Bogor 16680, Indonesia, E-mail: email@example.com
This investigation is aimed at evaluating the polyphenol profile, antioxidant, α-glucosidase and α-amylase inhibitory and the hypoglycemic activities of Acalypha hispida leaf extract in vivo. Powdered Acalypha hispida leaves were macerated with 70 and 96 % ethanol and decocted in distilled water. The filtrates were evaporated to obtain dry extracts. The extracts were found to have a polyphenol profile (ultraperformance liquid chromatography quadrupole time-of-flight) and to exhibit antioxidant activity (2,2-diphenyl-1-picrylhydrazyl) and α-glucosidase and α-amylase inhibition (spectrophotometer) in vitro and a hypoglycemic effect in vivo. This study revealed that the number of polyphenol subclasses of the 96 % ethanol extract was greater than that of the other extracts. The 70 and 96 % ethanol and aqueous extracts exhibited strong antioxidant activity (IC50<10 µg/ml). The 96 % ethanol extract showed very high inhibition of α-glucosidase and α-amylase activities. A hypoglycemic assay of the 96 % ethanol extract at 300 mg/kg dose exhibited a significant hypoglycemic activity in experimental rats. The present results suggested that the ethanol extract of Acalpha hispida leaves could be an effective hyperglycemic agent acting through inhibiting α-glucosidase and α-amylase.