Abstract
Protective and Therapeutic Potential of a Novel Phytobiological Formulation of Nutrients in the Treatment of Indomethacin-Induced Gastric Ulcer
King Fahd Medical Research Center, King Abdulaziz University, Jeddah 21589, 1Family and Community Medicine Department, Faculty of Medicine in Rabigh, King Abdulaziz University, Jeddah, Saudi Arabia, 2Department of Zoology, Molecular Cell Biology Laboratory, Faculty of Science, Assiut University, Assiut 71516, Egypt, 3Department of Physiology, Biochemistry and Pharmacology, Faculty of Veterinary Medicine, University of Jos, Jos, Plateau 930105, Nigeria, 4Department of Anatomy, Faculty of Medicine, King Abdulaziz University, Jeddah, 5Clinical Nutrition Department, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah 21589,Saudi Arabia, 6Vaccines and Immunotherapy Unit, King Fahd Medical Research Center, 7Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences,King Abdulaziz University, Jeddah 21589,Saudi Arabia,8Department of Clinical Microbiology and Immunology, Faculty of Medicine, King Abdulaziz University, Jeddah 21589, Saudi Arabia, 9Dr. Rath Research Institute San Jose, California 95138, United States of America, 10Center for Advanced Imaging, Faculty of Science, The University of Queensland, St Lucia QLD 4072, Australia
Correspondence Address:
S. Harakeh, King Fahd Medical Research Center, King Abdulaziz University, Jeddah 21589, Saudi Arabia, E-mail: sharakeh@gmail.com
Gastric ulcers have been considered as a major problem worldwide. This study investigated the protective synergistic effect of a novel nutrient formulation for the protection and treatment of indomethacin-induced gastric ulcer in rat model. 24, 8 w old male rats with a body weight between 220 and 240 g, were divided into four groups and used for this study. Group 1 consists of healthy controls and group 2 consists of ulcerative animals, group 3 consists of ulcerative+phytobiological formulation treatment (80 mg/kg) and group 4 consists of omeprazole (40 mg/kg). Pre-treatment with 80 mg/kg of phytobiological formulation improved the activities of the measured antioxidant enzymes, decreased lipid peroxidation and inflammation, as evidenced by improved surface and glandular epithelium (white arrows) of phytobiological treated group stained with hematoxylin and eosin and tumor necrosis factor alpha immuno-expression when compared with the ulcer group. In conclusion, the mechanism by which this phytobiological formulation prevents indomethacininduced gastric ulcer may be mediated via improving the homeostasis of enzymatic (superoxide dismutase) and nonenzymatic (glutathione) antioxidants, inhibiting (malondialdehyde) and decreasing the formation of inflammatory cytokines (tumor necrosis factor alpha, C-reactive protein content and interleukin-10). Consequently, this phytobiological formulation may be a beneficial nutrient formulation therapy for patients diagnosed with gastric ulceration.
