Abstract

The present study investigated the phytochemical, total phenolic, flavonoid content and in vitro antioxidant of the methanolic extract of the leaf of Ipomoea hederifolia Linn. In vitro antioxidant activity was determined using 2,2-diphenyl-1-picrylhydrazyl, hydroxyl radical, reducing power and phosphomolybdenum method. The total phenolic and total flavonoid contents were also determined using Folin Ciocalteau’s and aluminium chloride method respectively. The preliminary phytochemical screening showed the presence of alkaloids, glycosides, steroids, triterpenoids, flavonoids, phenols and tannins. Results indicated that the total phenolic and flavonoid content were found to be 101.28±0.9238 mg of gallic acid equivalents/g and 14.91±0.672 quercetin equivalent/g respectively. The half-maximal inhibitory concentration values of the extract based on the 2,2-diphenyl-1-picrylhydrazyl was 20.33 µg/ml as compared to standard (ascorbic acid) which is 18.30µg/ml while the half-maximal inhibitory concentration values of the methanolic extract of Ipomoea hederifolia and standard (ascorbic acid) for hydroxyl scavenging activity were found to be 104.9 µg/ml and 16.88 µg/ml respectively. The reducing power of methanolic extract and standard (ascorbic acid) at 25 µg/ml were found to be 0.183±0.002 and 0.396±0.001 respectively and the total antioxidant capacity of methanolic extract and standard (ascorbic acid) at 100 µg/ml and were found to be 0.094±0.0002 and 0.368±0.002 respectively.