All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Abstract

Quercetin Regulates Inflammatory Response of Retinal Ganglion Cells by Affecting the Tumor Necrosis Factor Receptor Associated Factor 6/Transforming Growth Factor-β-Activated Kinase-1 Signaling Pathway

Author(s): Zifeng Deng and Shibo Xiong*
Department of Ophthalmology, Hunan Children's Hospital, Changsha 410007, Hunan Province, 1Wuhan Eyegood Ophthalmic Hospital, Wuhan 430015, Hubei Province, China

Correspondence Address:
Shibo Xiong, Wuhan Eyegood Ophthalmic Hospital, Wuhan 430015, Hubei Province, China, E-mail: 8113141@qq.com


To investigate the protective effect of quercetin on the lipopolysaccharide-induced inflammatory response of retinal ganglion cell line 5 cells and to explore its possible molecular mechanism. Retinal ganglion cell line 5 cells were cultured in vitro and assigned into blank control group, lipopolysaccharide (1.0 μg/ml) induction group, lipopolysaccharide+low dose (0.25 μg/ml) quercetin group, lipopolysaccharide+medium dose (0.5 μg/ml) quercetin group and lipopolysaccharide+high dose (1.0 μg/ml) quercetin group. The relative survival rate of retinal ganglion cell line 5 cells was determined by cell counting kit-8 assay and the levels of inflammatory factors interleukin-6 and tumor necrosis factor-alpha were detected using enzymelinked immunosorbent assay kits. In addition, the levels of tumor necrosis factor receptor-associated factor 6, phosphorylation-transforming growth factor-beta-activated kinase 1 and transforming growth factorbeta- activated kinase 1 were measured by western blotting. The results of cell counting kit-8 assay revealed that quercetin could dramatically elevate the relative survival rate of retinal ganglion cell line 5 cells. The levels of inflammatory factors interleukin-6 and tumor necrosis factor-alpha in different quercetin groups were significantly lower than those in lipopolysaccharide induction group and the levels of interleukin-6 and tumor necrosis factor-alpha in cell supernatant declined with the increase of quercetin concentration in a concentration-dependent manner. In addition, the expression levels of tumor necrosis factor receptorassociated factor 6 and phosphorylation-transforming growth factor-beta-activated kinase 1 in different quercetin groups were remarkably lower than those in lipopolysaccharide induction group and they also declined with the increase of quercetin concentration in a concentration-dependent manner. Quercetin displays a protective effect against the lipopolysaccharide-induced inflammatory response of retinal ganglion cell line 5 cells and its mechanism is related to suppression of the activation of the tumor necrosis factor receptor-associated factor 6/transforming growth factor-beta-activated kinase 1 signaling pathway.

Full-Text | PDF

 
 
Google scholar citation report
Citations : 69022

Indian Journal of Pharmaceutical Sciences received 69022 citations as per google scholar report