All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.


Radical Scavenging and Endogenous Defence System Inducing Activities of 5-[(4-Chlorophenoxy)methyl]-1,3,4-oxadiazole-2-thiol: A Novel Antioxidant

Author(s): N. Shehzadi1*, K. Hussain, M. T. Khan1, N. I. Bukhari, M. Islam, M. Salman, S. Qamar, A. A. Iqbal, S. Z. Siddiqui2, A. Rehman2 and M. A. Abbasi2
Punjab University College of Pharmacy, University of the Punjab, Allama Iqbal Campus, Lahore-54000, 1The University of Lahore, 1-Km, Defence Road (Off Raiwind Road), Bhobatian Chowk, 2Department of Chemistry, Government College University, Lahore-54000, Pakistan

Correspondence Address:
The University of Lahore, 1-Km, Defence Road (Off Raiwind Road), Bhobatian Chowk, Lahore-54000, Pakistan, E-mail:;

5-[(4-Chlorophenoxy)methyl]-1,3,4-oxadiazole-2-thiol owing to the presence of –SH group is expected to have a significant reducing potential, which could be translated into antioxidant activity and to prove this, the present study explored the antioxidant potential and binding pattern of this compound to oxidative stress-related protein targets. The antioxidant properties were determined using in vitro methods with ascorbic acid and butylated hydroxytoluene as standards while interactions of 5-[(4-chlorophenoxy)methyl]-1,3,4-oxadiazole-2-thiol with protein tyrosine kinase 2-β and glutathione reductase were determined using online software, Mcule 1-Click Docking, 3DLigandSite and COACH. The antioxidant activity of 5-[(4-chlorophenoxy)methyl]-1,3,4-oxadiazole-2-thiol was found to be 89.30±0.013, 81.20±0.002, 80.52±0.016, 54.81±0.007, 52.87±0.008, 34.44±0.019 and 19.91±0.014 % in hydrogen peroxide scavenging assay, 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay, phosphomolybdenum assay, nitric oxide scavenging assay, reducing power assay, ferric thiocyanate assay and β-carotene bleaching assay, respectively. In all these assays, EC50 of 5-[(4-chlorophenoxy)methyl]-1,3,4-oxadiazole-2-thiol ranged from 0.32-0.93 mg/ml. The docking results indicated excellent binding to protein tyrosine kinase 2-β and glutathione reductase, maximum for the latter. The results of the present study revealed that 5-[(4-chlorophenoxy)methyl]-1,3,4-oxadiazole-2-thiol has the propensity to abrogate oxidation by inducing endogenous defence system and preventing radical chain reactions, hence might be considered a potential antioxidant for further investigations.

Full-Text | PDF

Google scholar citation report
Citations : 66710

Indian Journal of Pharmaceutical Sciences received 66710 citations as per google scholar report