Abstract

To investigate the effects of radix Ranunculus ternatus saponins on sarcoma osteogenic-2 cell malignancy and its possible mechanism. Sarcoma osteogenic-2 cells were treated with different doses (50, 100, 200 mg/l) of radix Ranunculus ternatus saponins for 24 h. Effect of radix Ranunculus ternatus saponins on sarcoma osteogenic-2 cell malignant behaviors were detected by cell counting, colony formation, wound-healing, and transwell invasion assays. Protein levels were detected by Western blotting. Treatment with radix Ranunculus ternatus saponins repressed the proliferative, migratory, and invading capacities of sarcoma osteogenic-2 cells in a dose-dependent manner. Radix Ranunculus ternatus saponins reduced neural-cadherin protein levels and elevated epithelial-cadherin protein levels. MicroRNA-1305 was downregulated in osteosarcoma samples, while microRNA-1305 was elevated in microRNA-1305 treated sarcoma osteogenic-2 cells. Upregulation of microRNA-1305 restrained sarcoma osteogenic-2 cell proliferation, metastasis, and invasion, while microRNA-1305 knockdown radix Ranunculus ternatus saponins-mediated inhibiting effects on sarcoma osteogenic-2 cell malignant behaviors. Radix Ranunculus ternatus saponins might inhibit sarcoma osteogenic-2 cell malignant behaviors by up-regulating miR-1305, which has a potential value for osteosarcoma treatment.